A    1        2   B      1     2    A. Entamoeba histolytica   Fixation according to Karnowsky=2%PFA + 2.5%GA    in Na-Cacodylat buffer   1) Pre-immune serum a 170 1:500 dil.   2) Antiserum a 170 1:500 dil.   Marked with protein A gold 10 nm, 1:20 dil.   Contrasting with Uranylacetat and lead citrate.   Magnification: 41,000  B. Leishmania d.   Fixation:4% PFA + 0.025%GA in Na-Cacodylat buffer   1) Pre-immune serum a 83 1:2,000 dil.   2) Antiserum a 83 1:2,000   Marked with Colloidal gold anti-chichem IgI 12 nm, 1:50 dil.   Contrasting with Uranylacetat and leas citrate.   Magnification: 41,000

Comments     In 1996 the following tasks have been carried out:  - The project “Microsporidiosis” pursued since 1993 in cooperation with the group Kock/Dr. Schottelius, Parasitology Section, and Dr. Sobottka, University Hospital Eppendorf, Hamburg, was continued. Diagnosis and species proof of Entamoeba histolytica in stool and biopsy specimens by pre-embedding were performed.  - Various proteins of E. histolytica could be localized by immuno electronmicroscopy using pre-embedding staining.  - Morphological proof of apoptosis of HL 60 cells after incubation with actinomycin and of nekrosis after incubation with E. histolytica.  - An embedding method with LR White for immuno electron microscopic studies of sections of amoeba, leishmania, microsporidia and T cells has been established.  - The Confocal Scanning Microscope Leica TCS NT was installed.  - 60 diagnoses for the Division of Clinical Medicine for Herpes virus, enterovirus, and microsporidia were performed.  - The participation in a quality control study “Standardization of electronmicroscopic virus diagnosis (Rapid Viral Diagnosis)” of the Robert Koch Institute, Berlin, as a reference laboratory has been continued.    Christel Schmetz     Herbert Schmitz