October 24th 2003

Department of Virology of the Bernhard-Nocht Institute for Tropical Medicine, Hamburg
Germany


For more information please contact Dr. Christian Drosten, Bernhard-Nocht-Institute, Hamburg
E-mail: drostenbni-hamburg.de

Please note that optimum concentrations of Magnesium ions (1-4 mM) and primers (100-300 nM) depend on reagents used and have to be optimized by end user!

NPconS2
ACCCCAATCAAACCAACgTAgTg

NPconAs1 (reverse)
CggTAgTAgCCAATTTggTCATC

Amplicon: 28134-28385 on Urbani strain